منابع مشابه
Presence of a Haloarchaeal Halorhodopsin-Like Cl− Pump in Marine Bacteria
Light-driven ion-pumping rhodopsins are widely distributed among bacteria, archaea, and eukaryotes in the euphotic zone of the aquatic environment. H+-pumping rhodopsin (proteorhodopsin: PR), Na+-pumping rhodopsin (NaR), and Cl--pumping rhodopsin (ClR) have been found in marine bacteria, which suggests that these genes evolved independently in the ocean. Putative microbial rhodopsin genes were ...
متن کاملMechanism of a molecular valve in the halorhodopsin chloride pump.
Halorhodopsin is a light-driven chloride anion pump in which the trans-->cis photoisomerization of a retinal chromophore triggers a photocycle resulting in the translocation of chloride across the plasma membrane. The mechanism of chloride transfer past the cis retinal is determined here by computing multiple pathways for this process. The calculations reveal two conditions of the valve mechani...
متن کاملHeterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl- pump activity.
Natronomonas pharaonis halorhodopsin (pHR) is an archaeal rhodopsin functioning as an inward-directed, light-driven Cl- pump. To characterize the electrophysiological features of the Cl- pump activity of pHR, we expressed pHR in Xenopus laevis oocytes and analyzed its photoinduced Cl- pump activity using the two-electrode voltage-clamp technique. Photoinduced outward currents were observed only...
متن کاملSpectral tuning in halorhodopsin: the chloride pump photoreceptor.
The spectral tuning of halorhodopsin from Halobacterium salinarum (shR) during anion transport was analyzed at the molecular level using DFT-QM/MM [SORCI+Q//B3LYP/6-31G(d):Amber96] hybrid methods. Insights into the influence of Cl(-) depletion, Cl(-) substitution by N3(-) or NO3(-), and mutation of key amino acid residues along the ion translocation pathway (H95A, H95R, Q105E, R108H, R108I, R10...
متن کاملFunctional reconstitution of halorhodopsin. Properties of halorhodopsin-containing proteoliposomes.
A one-step purification method for halorhodopsin was developed. Functional proteoliposomes were prepared from this preparation using cholate, which is removed by dialysis in the presence of asolectin or the polar halobacterial lipids. Light-induced outward directed transport of chloride by halorhodopsin was followed by measuring passive proton efflux in the presence of uncoupler; initial rates ...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 1987
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.27.13